Titlebook: Human Cell Culture Protocols; Joanna Picot Book 2005Latest edition Humana Press 2005 genes.phorbol ester.regulation.reproducible technique

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Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation,ity (.). The markers of mature articular chondrocytes are type II collagen (COL2A1), other cartilage-specific collagens IX (COL9) and XI (COL11), the large aggregating proteoglycan aggrecan, and link protein (..). Chondrocytes also synthesize a number of small proteoglycans such as biglycan and deco

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Condense

Cell Cultures of Autopsy-Derived Fibroblasts,eriments, which would help to elucidate the degenerative processes, cannot be performed with affected tissue. On the other hand, biopsy-derived human brain tissue from patients, as an alternative source for living cell material, is rare and, in autopsy-derived tissue, neuronal cells are generally al

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Purification of Peripheral Blood Natural Killer Cells,r lineages. NK cells are a discrete lymphocyte subset distinct from B and T cells based on both physical and phenotypic characteristics that can be exploited for their purification. Techniques based on differential cell buoyancy [centrifugation on discontinuous density gradients such as Percoll (.)]

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Human Fetal Brain Cell Culture,lls. Because of its cellular complexity, it is important to develop technical approaches that allow isolation and study of nerve and glial cells under conditions in which their environment can be precisely manipulated. Such methods have proven valuable in discovering the cellular and molecular mecha

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Culturing Human Schwann Cells,d methods used with other species (e.g., rat), simple dissociation of human peripheral nerve tissue has been found to result in both very low cell yields and poor Schwann cell purity (.,.). This is thought to be caused by the increased amount of connective tissue within human nerves and the mechanic

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Isolation and Culture of Human Alveolar Epithelial Cells,lium differ drastically from those of the more distal, alveolar epithelium. This chapter will concentrate on the isolation and culture of human alveolar epithelial cells that line the peripheral gas exchange region of the lung. Whereas immortalized cell lines emanating mostly from the different cell

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