Titlebook: Homologous Recombination; Methods and Protocol Andrés Aguilera,Aura Carreira Book 2021 Springer Science+Business Media, LLC, part of Spring

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Biochemical Analysis of D-Loop Extension and DNA Strand Displacement Synthesis, forks and eroded telomeres are restored. The central step in homologous recombination is homology-dependent pairing between a single-stranded DNA tail with an intact duplex molecule to generate a displacement-loop (D-loop), followed by DNA synthesis within the D-loop platform. This chapter describe

Occlusion

Resistance

Monitoring Homologous Recombination Activity in Human Cells,(HR), operating only in S/G2 cell-cycle phases and nonhomologous end joining (NHEJ) which is operative throughout the cell cycle. Because HR is a template-directed repair, it is generally less prone to errors and/or translocations than NHEJ..The HR pathway involves several effector proteins and regu

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Culmination

Branch Migration Activity of Rad54 Protein,ctions with high specificity and promotes their branch migration in an ATP hydrolysis-dependent manner. Here we describe the methods our laboratory used to characterize the branch migration activity of Rad54. These assays are applicable for other branch migration proteins regardless of whether they

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Holliday Junction Resolution,he reciprocal exchange of single strands between two DNA molecules. Given their ability to effectively bridge two sister chromatids or homologous chromosomes, cells have implemented various pathways to ensure their timely removal. One of them is the nucleolytic processing of the Holliday junctions b

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Intrachromosomal Recombination in Yeast,regions of the genome that are not repetitive, spontaneous recombination rates are too low to be detected by simple screening and require reporters where a recombination product can be selected. This chapter describes commonly used types of reporters where a gene is duplicated as direct repeats and

inundate

Genome-Wide Analysis of Mitotic Recombination in Budding Yeast,athway that guides the repair reaction by using a homologous DNA template. The budding yeast . is an excellent model system with which to study this repair mechanism and the resulting patterns of genomic change resulting from it. In this chapter, we describe an approach that utilizes whole-genome se