Titlebook: Histology Protocols; Tim D. Hewitson,Ian A. Darby Book 2010 Humana Press 2010 Histochemistry.Histological material.Imaging techniques.Labo

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Double Immunohistochemistry with Horseradish Peroxidase and Alkaline Phosphatase Detection Systemsol and describe necessary controls; additionally, we discuss common problems associated with this methodology, and detail troubleshooting recommendations. Although this method has been optimized for liver sections, it may be applicable for performing double immunostaining in a variety of tissue samples.

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Histology Protocols978-1-60327-345-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Tim D. Hewitson,Ian A. DarbyProvides an easily accessible reference volume for histological techniques written by leading researchers in the field.Details the techniques for investigating the expression of many different types o

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Tissue Preparation for Histochemistry: Fixation, Embedding, and Antigen Retrieval for Light Microscohistochemistry, these techniques have allowed us to better understand tissue and organ biology in situ. Success with each of these methods is dependent on the adequate preparation of material. In this article, we describe the basic steps required to prepare tissue for routine histochemical analysis.

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An Optimized RNA Extraction Method from Archival Formalin-Fixed Paraffin-Embedded Tissueiagnostic histopathological examination. Gene expression profiles of FFPE can provide insights into molecular mechanisms of disease but are difficult due to the high level of cross-linking of biomolecules by formalin fixation. Despite advances in molecular technologies, the quality of RNA obtained f

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Laser-Capture Microdissection and Pressure Catapulting for the Analysis of Gene Expression in the Reon techniques enabling sampling of specific cell populations or structures within the kidney. Laser-capture microdissection and pressure catapulting is a relatively new, entirely non-contact microdissection technique that facilitates the assay of mRNA and protein expression in single nephron segment