| 書目名稱 | High Throughput Protein Expression and Purification | | 副標題 | Methods and Protocol | | 編輯 | Sharon A. Doyle | | 視頻video | http://file.papertrans.cn/427/426533/426533.mp4 | | 概述 | Provides step-by-step protocols for protein production that utilize a wide range of cutting-edge techniques.Covers cell-based and cell-free methodologies needed to produce bacterial proteins, eukaryot | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | .Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In .High Throughput Protein Expression and Purification: Methods and Protocols., leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of?E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology? series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls. ..Cutting-edge and comprehensive, .High Throughput Protein Expression and Purification: Methods and Protocols. is an i | | 出版日期 | Book 2009 | | 關鍵詞 | Cloning; Escherichia coli; Genome sequencing; PCR; Parallel processing; membrane proteins; molecular biolo | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-59745-196-3 | | isbn_softcover | 978-1-61737-821-8 | | isbn_ebook | 978-1-59745-196-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2009 |
The information of publication is updating
|
|
|Archiver|手機版|小黑屋|
派博傳思國際
( 京公網(wǎng)安備110108008328)
GMT+8, 2025-10-7 14:29
發(fā)表于 2025-3-21 17:55:02
