Titlebook: High Content Screening; A Powerful Approach Paul A. Johnston,Oscar J. Trask Book 2018Latest edition Springer Science+Business Media LLC 20

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High Content Positional Biosensor Assay to Screen for Compounds that Prevent or Disrupt Androgen Recf castration resistant prostate cancer (CRPC). This chapter describes the implementation of an AR-TIF2 protein-protein interaction (PPI) biosensor assay to screen for small molecules that can induce AR-TIF2 PPIs, inhibit the DHT-induced formation of AR-TIF2 PPIs, or disrupt pre-existing AR-TIF2 PPIs

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High Content Imaging Assays for IL-6-Induced STAT3 Pathway Activation in Head and Neck Cancer Cell Lciated Janus kinase (JAK) phosphorylates STAT3 at Y705. pSTAT3-Y705 dimers traffic into the nucleus and bind to specific DNA response elements in the promoters of target genes to regulate their transcription. However, IL-6 receptor activation induces the phosphorylation of both the Y705 and S727 res

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Utilization of Multidimensional Data in the Analysis of Ultra-High-Throughput High Content Phenotypiion provided by these screens, a combination of traditional as well as nontraditional hit identification and prioritization strategies is required. Here, we describe the workflow and analytics of multidimensional high content data to differentiate, group, and prioritize hits.

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.Antigen Processing: Methods and Protocols., expert researchers in the field provide a comprehensive set of protocols for studying presentation of antigens produced in the standard processing pathways for MHC class I and class II molecules. The chapters follow chronology of intracellular processing

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Roy Edwardal for reproducible results.Contains key notes and implement.Antigen processing is a biological process that prepares antigens for the presentation to special cells in the immune system called T lymphocytes. In .Antigen Processing: Methods and Protocols., expert researchers in the field provide a co

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