Titlebook: Heterologous Protein Production in CHO Cells; Methods and Protocol Paula Meleady Book 2017 Springer Science+Business Media LLC 2017 CRISPR.

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,Strategies and Considerations for Improving Expression of “Difficult to Express” Proteins in CHO Ceg the expression bottleneck requires troubleshooting techniques specific for the given molecule and host. The complex array of intracellular processes involved in protein expression includes transcription, protein folding, post-translation processing, and secretion. Challenges in any of these steps

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Glycoengineering of CHO Cells to Improve Product Quality,oteins, especially glycoproteins. These glycoproteins include oligosaccharide or glycan attachments that represent one of the principal components dictating product quality. Especially important are the N-glycan attachments present on many recombinant glycoproteins of commercial interest. Furthermor

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Anti-Apoptosis Engineering for Improved Protein Production from CHO Cells,t production of therapeutic proteins. By establishing stable cell lines that overexpress anti-apoptotic genes or down-regulate pro-apoptotic genes, the final product yields can be enhanced as cells become more resistance to environmental stresses. From the selection of high-expressing clones to veri

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Conditional Knockdown of Endogenous MicroRNAs in CHO Cells Using TET-ON-, Sponge Vectors,rable phenotype in the biotech industry. The use of constitutively expressed “miRNA sponge” vectors in which multiple, tandem miRNA binding sites containing transcripts are transcriptionally regulated by a constitutive promoter for down regulating the levels of endogenous microRNAs in Chinese hamste

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