Titlebook: Heterologous Gene Expression in E.coli; Methods and Protocol Thomas C. Evans, Jr.,Ming-Qun Xu Book 2011 Springer Science+Business Media, LL

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Semi-synthesis of Glycoproteins from ,Through Native Chemical Ligation,menable to total chemical synthesis. These fragments of defined sequence and structure can be elaborated to full-length proteins through NCL reactions with suitable components derived from bacterial origin. This report describes methods for the bacterial production of components for NCL and their use in typical reactions.

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