Titlebook: Hematopoietic Stem Cell Protocols; Christopher A. Klug,Craig T. Jordan Book 20021st edition Humana Press 2002

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Long-Term Culture-Initiating Cell Assays for Human and Murine Cells,ules that influence their cell-cycle status, viability, motility, and differentiation. These include components of the extracellular matrix, soluble and bound growth-promoting factors and inhibitors, and adhesion molecules that mediate direct interactions between cells. The long-term culture (LTC) s

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Quantitation of Murine and Human Hematopoietic Stem Cells by Limiting-Dilution Analysis in Competit to be measured which must be incorporated into the assay design, and the end points to allow its specific detection. The most primitive hematopoietic stem cells (HSC) in mouse and man are characterized by two cardinal properties that distinguish them from more mature clonogenic cells and their term

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Ex Vivo Expansion of Human and Murine Hematopoietic Stem Cells,t of practical assays for quantitating highly primitive hematopoietic cells. This progress has now made feasible the predictable manipulation of hematopoietic stem cells (HSC) and progenitors for a variety of experimental and clinical applications. Nevertheless, our understanding of events that indu

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Genetic Modification of Murine Hematopoietic Stem Cells by Retroviruses,ration of a transgene with high efficiency. Pioneering work by a number of groups has demonstrated the feasibility of using this method for gene transfer to primitive, multipotential long-term repopulating hematopoietic stem cells (HSC) (.–.). In the case of the hematopoietic system, it is required

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Retroviral Transduction of FACS-Purified Hematopoietic Stem Cells,ve hematopoietic stem cells (HSC) (.,.). During this period, both vectors and packaging systems used to generate virus have undergone considerable modification. This has led to increased production of high-titer, replication-defective retrovirus that is more resistant to gene inactivation following

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Retroviral-Mediated Transduction and Clonal Integration Analysis of Human Hematopoietic Stem and Premia (MoMuLV)-based retroviral vectors. MoMuLV-based vectors have the ability to efficiently transfer genes into mammalian cells, leading to permanent integration of a single copy of the gene of interest into the cellular chromosomes. The technique of single-colony inverse [polymerase chain reaction