Titlebook: Helicases; Methods and Protocol Mohamed M. Abdelhaleem Book 2010 Humana Press 2010 DNA.Escherichia coli.RNA.Termination.genes.molecular bio

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Protein Displacement by Helicases,cid and unwind the nucleic acid (DNA and RNA). ATP hydrolysis provides energy for helicase translocation and unwinding. The unwinding process provides ssDNA intermediates necessary for replication, recombination, and repair. Mutations in specific DNA helicases can lead to disruption in DNA metabolis

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Simple Enzymatic Assays for the In Vitro Motor Activity of Transcription Termination Factor Rho fro.ilization) regions of nascent RNA transcripts. Once bound to the RNA chain, Rho uses ATP as an energy source to produce mechanical work and disruptive forces that ultimately lead to the dissociation of the ternary transcription complex. Although transcription termination assays have been useful to