Titlebook: Hedgehog Signaling Protocols; Natalia A. Riobo Book 2015Latest edition Springer Science+Business Media New York 2015 Cell Culture.Cytoneme

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Determination of Acetylation of the Gli Transcription Factors, status of these proteins represents a useful marker to monitor Hh activation in pathophysiological conditions. Herein we describe the techniques utilized to detect in vitro and intracellular acetylation of the Gli transcription factors.