Titlebook: Hedgehog Signaling; Methods and Protocol Xiaochun Li Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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An In Situ Fluorescence Assay for Cholesterol Transporter Activity of the Patched,. This ratiometric fluorescence technique allows real-time monitoring of the cholesterol transporter activity of plasma membrane-resident proteins in living cells. When applied to the hedgehog signaling system, it enables direct quantitative measurement of the cholesterol transporter activity of Pat

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The Immunofluorescence-Based Detection of Hedgehog Pathway Components in Primary Cilia of Cultured ugh primary cilia affect Hedgehog signaling, and many Hedgehog pathway components traffic through or accumulate in cilia. This protocol provides methods for immunofluorescence staining of cilia-accumulated Hh pathway components, such as Smoothened, in cultured NIH 3T3 cells.

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,Expression, Purification, and Structure Determination of Human PTCH1–HH-N Complexes,PTCH1 are implicated in many human cancers. Structural investigation revealed the mechanism of PTCH1-mediated HH signal regulation, further facilitating the therapeutic development of cancers. Here, we describe the expression and purification of a nearly full-length functional PTCH1 variant, PTCH1*.

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