Titlebook: Handbook of Single-Molecule Biophysics; Peter Hinterdorfer,Antoine Oijen Book 2009 Springer-Verlag New York 2009 Biomembran.Biophysics.FRE

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Single-Molecule Imaging in Live Cells,ich are the most widely used fluorescent labels for live-cell imaging. The chromophore structures and the associated photochemical and photophysical properties of fluorescent proteins are discussed in detail, with a particular focus on how they influence single-molecule imaging in live cells. A few

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Fluorescence Correlation Spectroscopy in Living Cells,cromolar concentration of fluorescent molecules in solution. As primary parameters, FCS provides time constants of the fluorescence emission, as well as numbers and dwell times of the observed particles diffusing through the open volume. A biochemical reaction or macromolecular rearrangement causing

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Precise Measurements of Diffusion in Solution by Fluorescence Correlations Spectroscopy,-focus FCS. It explains the general optical setup of an FCS system, data acquisition, and data analysis. It discusses numerous potential optical and photophysical artifacts of FCS measurements and explains how these problems are circumvented by dual-focus FCS.

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Nanopores: Generation, Engineering, and Single-Molecule Applications,iological protein pore, electrical recordings are now routinely performed with synthetic pores sculptured into polymeric and inorganic membranes. Assisted by channel engineering, the range of analytes has been expanded from nucleic acids to peptides, proteins, organic polymers, and small molecules.

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Single-Molecule Manipulation Using Optical Traps,Consisting essentially of a strongly focused light beam, optical traps were first developed and demonstrated in the 1970s and 1980s by Arthur Ashkin and colleagues (Ashkin et al. 1986). These early pioneers showed that micron-sized dielectric particles could be held and manipulated in solution by us