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Titlebook: Handbook of Biological Confocal Microscopy; James B. Pawley Book 1990 Springer Science+Business Media New York 1990 biology.cell.developme

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發(fā)表于 2025-3-30 11:58:51 | 只看該作者
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發(fā)表于 2025-3-30 13:56:28 | 只看該作者
Pneumothorax. Chronische Phthise,inition and the contrast of the image are better than with a standard microscope, the device is very well-suited for optical cross-sectioning, and with the use of a laser beam, the intensity of illumination can be very high. At the same time, it is possible to choose the wavelength of the illuminati
53#
發(fā)表于 2025-3-30 17:37:08 | 只看該作者
https://doi.org/10.1007/978-3-642-72041-3sectioning (Wilson and Sheppard, 1984). It is probably the latter property which is most useful as it gives rise to the ability to image a thick specimen in three-dimensions. This is possible because the optical system images information only from a thin region in the neighbourhood of the focal plan
54#
發(fā)表于 2025-3-30 22:53:44 | 只看該作者
https://doi.org/10.1007/978-3-642-97848-7ject plane in the absence of light scattered from neighboring points. In this respect confocal microscopy differs from conventional brightfield microscopy where the intent is to view an object under uniform field illumination. The detector in the disk-scanning confocal microscope of Petran, Hadravsk
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發(fā)表于 2025-3-31 03:10:02 | 只看該作者
56#
發(fā)表于 2025-3-31 08:11:15 | 只看該作者
https://doi.org/10.1007/978-3-662-13130-5main limitation of the wide-field microscope for 3D imaging. The confocal microscope rejects this out-of-focus light with the confocal pinhole and provides greater resolution than the wide-field microscope. This depth discrimination of the confocal microscope makes it attractive for 3D optical secti
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