Titlebook: Handbook of Biological Confocal Microscopy; James B. Pawley Book 19952nd edition Springer-Verlag US 1995 Confocal.Microscopy.Pawley.biolog

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Guiding Principles of Specimen Preservation for Confocal Fluorescence Microscopy, react with electron-dense stains deposited in fixed specimens. Immunogold labeling permits the study of non-electron-dense material, but EM sections must still be very thin to avoid problems with the penetration of the labeled antibodies and to reduce scattering of the electron beam.

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Foundations of Confocal Scanned Imaging in Light Microscopy,he new microscope, one can slice incredibly clean thin optical sections out of thick fluorescent specimens; view specimens in planes tilted to, and even running parallel to, the line of sight; penetrate deep light-scattering tissues; gain impressive three-dimensional (3D) views at very high resoluti

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Fundamental Limits in Confocal Microscopy,y the use of confocal sampling techniques. This chapter will discuss the fundamental considerations that limit the performance of all confocal microscopes. Though at present no commercially available equipment approaches these limits, some simple tests will be described to help the user assess how c

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The Specimen Illumination Path and Its Effect on Image Quality,n) optical paths contribute equally to the formation of the final image. However, many of the optical and imaging properties of confocal microscopes can be analyzed effectively by considering the illumination and detection paths to be two distinct entities. In this chapter, we explore one of these o

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