Titlebook: Hairy Root Cultures Based Applications; Methods and Protocol Vikas Srivastava,Shakti Mehrotra,Sonal Mishra Book 2020 Springer Nature Singap

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A Protocol for the Selection of Spontaneous Variants from Established , Hairy Root Cultures,ell division within complete tissues must occur in an organized manner to ensure the correct cell differentiation and identity, high genetic stability is frequently associated to organ in vitro culture, such as hairy roots. However, this stability could hamper the appearance of cell variants because

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Strategies for the Optimization of Culture Conditions for Increasing Metabolite Production Through of . (L.) G. Don, Madagascar periwinkle. The first step consists in culturing roots in an alkaloid production medium (APM) for 10?days before exposing them to 200?μM methyl jasmonate for up to 48?h. APM formulation is based on Gamborg’s B5 medium, at half of its ionic strength and vitamin concentra

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Tropane Alkaloid Production by the Establishment of Hairy Root Cultures of , and Elicitation,ompounds. Among them, hyoscyamine, anisodamine (6β-hydroxyhyoscyamine), and scopolamine are the most important ones from a therapeutical point of view. Although the chemical synthesis of these alkaloids is possible, its complexity, costs, and low quality of the compounds obtained make it a nonviable

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Elicitation as an Essential Strategy for Enhancing Anthraquinone Accumulation in Hairy Root Culturecing secondary metabolites due to its multiple advantages: production under controlled culture conditions; independence from geographic, seasonal or weather conditions; and since it avoids the use of land and the extraction from the natural source, it has a low environmental impact. Since the produc

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Establishment of Hairy Root Cultures for the Production of Biopharmaceuticals and Optimization of M stability, fast establishment of transgenic lines and fast biomass accumulation. In this chapter, we will describe the protocols used to obtain HR cultures from different plant species (., . and . subsp. .) based on . infection. These protocols have been successfully used to produce different heter