Titlebook: HPLC of Peptides and Proteins; Methods and Protocol Marie-Isabel Aguilar Book 2004 Humana Press 2004

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Reversed-Phase High-Performance Liquid Chromatographys a very powerful technique for the analysis of peptides and proteins because of a number of factors that include: (1) the excellent resolution that can be achieved under a wide range of chromatographic conditions for very closely related molecules as well as structurally quite distinct molecules; (

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Ion-Exchange Chromatography) separations can be fast, 3) in general, recoveries are high, 4) buffer components are nondenaturing and frequently compatible with further downstream chromatographic separation or assay systems, 5) process can be used as a concentration step, to recover proteins from a dilute solution. The disadva

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Liquid Chromatography-Mass Spectrometry and Tandem Mass Spectrometry of Peptides and Proteinsof peptides (or protein-derived peptides) in the mass spectrometer using the technique of collision-induced dissociation (CID). The fragments generated with CID all originate from the precursor; thus, supplementary information relating to the primary sequence and post-translational modifications of

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Multidimensional HPLC Purification of Proteins compatible with downstream analysis. Successful micropreparative HPLC requires minimal losses of both mass and biological activity during the chromatographic purification and other associated nonchromatographic sample manipulation (e.g., sample dilution, pH adjustment, storage, chemical manipulatio

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The Human Body and Weightlessnessmolecules. In particular, HPLC in its various modes has become the central technique in the characterization of peptides and proteins and has, therefore, played a critical role in the rapid advances in the biological and biomedical sciences over the last 10 years.

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https://doi.org/10.1007/978-3-642-14724-1arides) based on differences in their charge. IEX can be a highly selective chromatographic technique, being able to resolve, for example, proteins which differ by only a single charged group (.). The process relies upon the formation of ionic bonds between the charged groups on biomolecules (typica