Titlebook: HIV Reservoirs; Methods and Protocol Guido Poli,Elisa Vicenzi,Fabio Romerio Book 2022 Springer Science+Business Media, LLC, part of Springe

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U1 and OM10.1. Myeloid Cell Lines as Surrogate Models of Reversible Proviral Latencyould be used to investigate features of proviral reactivation, as reviewed in (Cassol et al. J Leukoc Biol 80:1018–1030, 2006), two cell lines in particular have been broadly used and will be here discussed: the U937-derived U1 and HL-60–derived OM-10.1.

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TGF-β Signaling Supports HIV Latency in a Memory CD4+ T Cell Based In Vitro Modely and reversion assay (LARA) culture conditions uniquely retain phenotypically and transcriptionally distinct memory CD4+ T cell subsets that allow in a single assay to assess LRA activity in each memory subset and differential examination of the dynamics of HIV latency reversal.

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An In Vitro System to Model the Establishment and Reactivation of HIV-1 Latency in Primary Human CD4original and a simplified version of HIV-1 latency models that mimics this process using replication competent viruses. Our model allows generation of large numbers of latently infected CD4+ T cell to dissect molecular mechanisms of HIV latency and reactivation.

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Experimental Models to Study HIV Latency Reversal from Male Genital Myeloid Cellscted by adapted viral outgrowth assays. Altogether, these approaches provide invaluable tools for the investigation on tissue-specific pathways that HIV-1 employs to reach host cells and form reservoirs in the genital mucosa. These models will contribute to the development of an efficient and targeted prophylaxis against HIV and of a HIV cure.

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https://doi.org/10.1007/978-1-0716-1871-4Primary non-CD4+ T lymphocyte; cervical tissue; animal models; QVOAS; TILDA

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HIV Reservoirs978-1-0716-1871-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

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