Titlebook: HIV Protocols; Vinayaka R. Prasad,Ganjam V. Kalpana Book 2016Latest edition Springer Science+Business Media New York 2016 HIV-1 RNA struct

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Vinayaka R. Prasad,Ganjam V. KalpanaIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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https://doi.org/10.1057/9781137537614 of production and imaging of dual-labeled HIV viral particles that allows the visualization of two events. Varying release of the intravirion fluid phase marker reveals virion fusion and the loss of the integrity of HIV viral cores with the use of live wide-field fluorescent microscopy.

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Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Vi of production and imaging of dual-labeled HIV viral particles that allows the visualization of two events. Varying release of the intravirion fluid phase marker reveals virion fusion and the loss of the integrity of HIV viral cores with the use of live wide-field fluorescent microscopy.

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https://doi.org/10.1007/978-1-4939-3046-3HIV-1 RNA structure; HIV-1 latency; HIV-1 replication; NeuroAIDS; RNA protein interactions; Virology

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Measuring T Cell-to-T Cell HIV-1 Transfer, Viral Fusion, and Infection Using Flow Cytometryuorescent virus particles, can be used to measure cell-to-cell transfer of virus particles. HIV NL-GI, a clone that expresses GFP as an early gene, facilitates the measure of productive infection after cell-to-cell contact. Lastly, a variation of the β-lactamase (BlaM)-Vpr fusion assay can be used t

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Novel Biochemical Tools for Probing HIV RNA Structureres of RNA, while probing strategies that utilize “through-space” cleavage reagents such as methidiumpropyl-EDTA (MPE) and peptides appended with an ATCUN (amino terminal copper/nickel binding motif) can provide insight into 3D organization. Combinational application of these techniques provides a f