Titlebook: HDAC/HAT Function Assessment and Inhibitor Development; Methods and Protocol Oliver H. Kr?mer Book 2017 Springer Science+Business Media New

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Analysis of HDACi-Induced Changes in Chromosomal Passenger Complex Localizationn tumor cells, thus leading to severe mitotic defects often followed by apoptosis. In this chapter, we describe how HDACi-induced changes of the CPC localization can be analyzed by indirect immunofluorescence using CPC-specific primary and fluorophore-coupled secondary antibodies followed by confocal microscopy.

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https://doi.org/10.1007/978-90-481-8969-4tionated cell populations could be used for further studies. In our laboratory, these protocols are routinely used to specifically investigate the molecular basis of histone acetylation/acylation-driven epigenetic programming.

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The Future of Orthopaedic Sports Medicinement procedure itself, the tumor growth surveillance, the explantation procedure, and finally the handling of obtained xenograft tissues are described step by step. This technical description is completed by numerous tips and alternatives designed to allow for an easy adaptation and transfer to other laboratories.

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