Titlebook: ;

ACRID

Working-Memory

Golden Gate Cloning of Multigene Constructs Using the Modular Cloning System MoClo, The assembly strategy is then defined following a set of standard rules. Multigene constructs are then assembled using a series of one-pot assembly steps with the set of identified parts and vectors.

Bravado

Misnomer

Automation and Miniaturization of Golden Gate DNA Assembly Reactions Using Acoustic Dispensers,ield of synthetic biology. The use of this technology allows miniaturization and parallelization of molecular reactions in a tip-free manner, making it sustainable by reducing plastic waste and reagent usage. Here, we provide a step-by-step protocol for performing and parallelizing Golden Gate cloning reactions in 1?μL total volume.

銀版照相

Combining Oligo Pools and Golden Gate Cloning to Create Protein Variant Libraries or Guide RNA Librwork within the Yeast Toolkit Golden Gate scheme, but are in principle compatible with any other Golden Gate-based modular cloning toolkit and extendable to other restriction enzyme-based cloning methods beyond Golden Gate. Our methods yield high-quality, affordable, in-house variant libraries.