Titlebook: Glycan Microarrays; Methods and Protocol Michelle Kilcoyne,Jared Q. Gerlach Book 2022 Springer Science+Business Media, LLC, part of Springe

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https://doi.org/10.1007/978-3-322-91637-2 the reverse-phase format where glycoprotein samples are spotted onto microarray slide and then are incubated with set of lectins. This configuration allows high-throughput screening of a large cohort of samples by a set of lectins without need of separation of glycans from glycoproteins. We applied

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https://doi.org/10.1007/978-3-663-10626-5 cells on glycan microarray is of interest. However, many whole mammalian cells are not compatible with glycomics microarray formats and instead cell-derived micelles are prepared and profiled instead of whole cells as they can accurately represent the parental cell glycome. In this chapter, we desc

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Neoglycoprotein and Glycoprotein Printing on a Hydrogel Functionalized Microarray Surface and Incubacells, and tissues. Glycan microarrays comprise a wide variety of platforms, differing in surface chemistry, presentation of carbohydrates, carbohydrate valency, and detection strategies, all of which impact on analyte performance. This chapter describes detailed methods for printing neoglycoprotein

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Evaluation of Glycan-Binding Specificity by Glycoconjugate Microarray with an Evanescent-Field Fluoralyzed by incubation with an array in which a series of glycans are immobilized. Various research groups in the world have developed glycan microarray. Among them, our glycan microarray has two unique points: one is the incorporation of the evanescent-field fluorescence detection system, and another

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Multiplex Glycan Bead Array (MGBA ) for High Throughput and High Content Analyses of Glycan-Binding ious glycan-binding proteins including anti-glycan antibodies. This platform takes advantage of the commercially available Luminex beads to construct glycan arrays that are easily customizable at will and anytime by researchers. The platform allows the simultaneous analyses of up to 500 glycans and

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Nanocube-Based Fluidic Glycan Arraysuch two-dimensional motion, some weakly binding glycans can participate in lectin binding processes, eventually changing lectin binding behaviors. This chapter discusses a plasmonic nanocube sensor that allows users to detect lectin binding kinetics in a cell membrane mimicking environment. This as

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