Titlebook: Glutamate Receptors; Methods and Protocol Corinna Burger,Margaret Jo Velardo Book 2019 Springer Science+Business Media, LLC, part of Spring

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Women, Sport and Adult Educationndary to an underlying neoplasm. Some of these paraneoplastic encephalitides express serum antibodies, but these were not thought to be pathogenic but instead have a T-cell-mediated pathophysiology. Over the last two decades, several pathogenic antibodies against neuronal surface antigens have been

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Single-Molecule FRET Methods to Study Glutamate Receptorses introduced on the protein of interest. This method is particularly attractive to study ion channel proteins as single-molecule current recordings have been used to study the function of these proteins for several decades. Here we describe the smFRET method used to study glutamate receptors.

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Ultrastructural Observation of Glutamatergic Synapses by Focused Ion Beam Scanning Electron Microscoture and function. Recent development of focused ion beam scanning electron microscopy (FIB/SEM) has made it possible to image neuronal structures with high speed and efficiency. Here, we present our routine protocol for correlative two-photon microscopy and FIB/SEM imaging of glutamatergic synapses

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Preparation of Organotypic Slice Cultures for the Study of Glutamate Receptor Functiongenetic manipulation of the receptors and regulatory signaling cascades as well as a more precise pharmacological intervention. We describe a method to prepare organotypic hippocampal slices that can be easily adapted to other brain regions. Brain slices are laid on porous membranes, and culture med