Titlebook: Glutamate Receptors; Methods and Protocol Corinna Burger,Margaret Jo Velardo Book 2019 Springer Science+Business Media, LLC, part of Spring

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Preparation of Synaptoneurosomes for the Study of Glutamate Receptor Functionus Percoll-sucrose density gradients, collection of brain tissue, preparation of brain homogenates, isolation of synaptoneurosome bands from the discontinuous Percoll-sucrose gradients, and radiolabeling SN proteins. De novo protein synthesis can be reproducibly measured in SN prepared by this method.

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https://doi.org/10.1057/9780333982990asuring metabotropic glutamate receptor-mediated long-term depression in hippocampal slices using extracellular field recordings and includes specific details for the application of this technique in the study of neuronal aging.

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Glutamate Receptor Probing with Rapid Application and Solution Exchange (RASE)s at the same set of receptors, thus allowing powerful paired-sample statistical approaches. In addition, the same experimental equipment fabricated for the ultra-resolution probing of receptor kinetics can also be applied in several other types of electrophysiological experiments.

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Induction of Metabotropic Glutamate Receptor-Mediated Long-Term Depression in the Hippocampal Schaffasuring metabotropic glutamate receptor-mediated long-term depression in hippocampal slices using extracellular field recordings and includes specific details for the application of this technique in the study of neuronal aging.

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Book 2019ultrastructure, receptor dynamics/receptor interactions and trafficking, cellular plasticity, receptor gene regulation, epigenetics, and clinical applications. In addition, it includes overview chapters that provide critical reviews and historical perspectives on particular techniques and fields of

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Learning to Belong in the Worldes introduced on the protein of interest. This method is particularly attractive to study ion channel proteins as single-molecule current recordings have been used to study the function of these proteins for several decades. Here we describe the smFRET method used to study glutamate receptors.

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Learning to Cooperate, Cooperating to Learnture and function. Recent development of focused ion beam scanning electron microscopy (FIB/SEM) has made it possible to image neuronal structures with high speed and efficiency. Here, we present our routine protocol for correlative two-photon microscopy and FIB/SEM imaging of glutamatergic synapses