Titlebook: Glucose Transport; Methods and Protocol Karin Lindkvist-Petersson,Jesper S‘Hansen Book 2018 Springer Science+Business Media LLC 2018 GLUTs.

移植

青石板

Expression and Purification of Rat Glucose Transporter 1 in ,, in shaker flasks, as commonly performed in academic research laboratories, results in relatively high yields of membrane protein. The purification protocol describes all steps needed to obtain a pure and homogenous GLUT1 protein solution, including cell growth, membrane isolation, and chromatographic purification methods.

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Banquet

Glucose Uptake in Heterologous Expression Systems,hrough a previously described radiolabeled glucose transport assay. Although this discussion is focused on the GLUT1 transporter, these techniques are easily transferrable to other glucose transporters.

novelty

Halfhearted

GLUT Characterization Using Frog , Oocytes,expression level by biotinylation and Western blotting analysis. Finally, we also describe how GLUT9-expressing oocytes can be used to measure urate kinetics by radioisotopes as well as two-microelectrode voltage clamping techniques.

BUOY

Alopecia-Areata

Applying Microfluidic Systems to Study Effects of Glucose at Single-Cell Level,ofluidic systems are fabricated and how they are utilized. Finally, we discuss how the large amount of data can be analyzed in a “semi-automatic” manner using custom-made software. In summary, we provide a guide to how to use microfluidic systems in single-cell studies.

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轉(zhuǎn)換

1064-3745 ation advice from the experts.Includes supplementary materiaThis volume explores methods used to examine the structure-function relationship of glucose transporters both in vitro and ex vivo. Chapters in this book cover topics such as expression and purification of glucose transporters in heterologo