Titlebook: Germline Stem Cells; Michael Buszczak Book 2017Latest edition Springer Science+Business Media New York 2017 Germline stem cell.primordial

Gerontology

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Visualization and Lineage Tracing of Pax7+ Spermatogonial Stem Cells in the Mouse, we describe the use of a monoclonal antibody to Pax7 as a means to detect spermatogonial stem cells (SSCs) both in tissue sections and in intact seminiferous tubules. Furthermore, we describe methods for lineage tracing as an alternative method to visualize Pax7. spermatogonial stem cells and their progeny.

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Musculoskeletal

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NEG

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Introduction to Flash Extensibility,echnical difficulties in isolating early larval ovaries. In addition, purifying RNA from larval ovaries proves to be more challenging than purifying it from other organs. Here we describe a technique for dissecting ovaries from early larvae and advise on how to extract RNA with maximum yield and pur

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https://doi.org/10.1007/978-1-4842-8170-3ls and their somatic cell neighbors can be identified at single-cell resolution within their native environment. Here we describe a fluorescent-based technique for the acquisition of 4D datasets of the . ovariole for periods that can exceed 12 consecutive hours. Live-cell imaging facilitates the inv