Titlebook: Germline Stem Cells; Methods and Protocol Michael Buszczak Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under e

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https://doi.org/10.1007/978-3-476-03131-0g the . GSC pool and early stages of germ cell differentiation. The methods include examination of germlines in living and fixed worms, cell cycle analysis, and analysis of markers. We also discuss assays to separate mutant phenotypes that affect the stem cell vs. differentiation decision from those that affect germ cell processes more generally.

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Cynthia Blomquist Gustavson MSW, ACSW, LCSWhe reliability of the results. Here, we provide our semi-optimized protocol of enrichment of?undifferentiated germ cells and somatic cells from non-tumorous?. testis, which we have successfully improved after multiple trials.

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Enrichment of Undifferentiated Germline and Somatic Cells from , Testes,he reliability of the results. Here, we provide our semi-optimized protocol of enrichment of?undifferentiated germ cells and somatic cells from non-tumorous?. testis, which we have successfully improved after multiple trials.

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,Lebensstile im sozial?kologischen Kontext, sibling GSCs. To facilitate the study of the cellular and molecular mechanisms underlying the process of germline dedifferentiation in the . testis, here we describe techniques for inducing high rates of dedifferentiation and for unambiguously labeling dedifferentiated GSCs.

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Spermatogonial Dedifferentiation into Germline Stem Cells in , Testes, sibling GSCs. To facilitate the study of the cellular and molecular mechanisms underlying the process of germline dedifferentiation in the . testis, here we describe techniques for inducing high rates of dedifferentiation and for unambiguously labeling dedifferentiated GSCs.