Titlebook: Germline Stem Cells; Methods and Protocol Michael Buszczak Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under e

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https://doi.org/10.1007/978-3-030-27492-4to build comprehensive cell-type-specific transcriptomic atlases for a wide range of tissues in several model organisms in order to discover cell-type-specific markers and drivers of gene expression. One such tissue is the ovary of the fruit-fly ., which is a popular model system with wide-ranging a

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Botanical Pesticides as Biocontrol Productsspecific regions in the gonads and can be identified because they uniquely express the . gene, which encodes a conserved regulator of translation. A method is presented here for identifying germline stem cells in the ovary and testis using a combined protocol for whole-mount fluorescent RNA in situ

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https://doi.org/10.1007/978-3-658-27172-5imordial germ cells (PGCs) that will generate the oogonia. First, these cells proliferate mitotically, and then they trigger the meiotic program and initiate meiotic prophase I. Since these processes happen during gestation, their study had been very limited and challenging. Recently, we reported th

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David Jou,José Casas-Vázquez,Georgy Lebonn of the dynamic changes occurring at the single-cell level. Here we describe methods for whole-mount immunofluorescence, clearing, imaging, and analysis of whole ovarian tissue in 3D throughout murine development and aging.

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David Jou,José Casas-Vázquez,Georgy Lebontudy germ cell-specific gene and protein expression, as well as determine direct effects of signaling molecules, it is necessary to prepare enriched populations of germ cells and maintain them in culture for several hours to multiple days. The protocols in this chapter are designed to provide a guid

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David Jou,José Casas-Vázquez,Georgy Lebonment. Consequently, methods to culture mammalian stem cells through spermatogenesis in defined systems have not been established. Lack of success at culturing mammalian stem cells through spermatogenesis in defined systems reflects an inability to experimentally recapitulate biochemical events that

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Extended Irreversible Thermodynamicsvelopment, limiting the ability to study fundamental developmental steps in human reproductive biology. However, recent advancements in generating in-vitro models of gametogenesis have allowed the field to generate human primordial germ cell-like cells (hPGCLCs). In this chapter, we will review the

TIGER

Extended Linear Chain Compounds PSCs with distinct properties. By modulating the FGF, TGF-β, and WNT pathways, we have derived intermediate PSCs (FTW-PSCs) that are permissive for direct primordial germ cell-like cell (PGC-LC) induction in vitro. Here, we describe the method for derivation and maintenance of mouse and human FTW-P

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978-1-0716-3261-1The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

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