Titlebook: Germline Development in the Zebrafish; Methods and Protocol Roland Dosch Book 2021 Springer Science+Business Media, LLC, part of Springer N

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Cryopreservation of Pooled Sperm Samples,s, and can prevent catastrophic loss of irreplaceable research lines. In this chapter, we present the sperm collection, dilution, cryopreservation, thawing, and in vitro fertilization procedures used at the Zebrafish International Resource Center (ZIRC).

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結(jié)構(gòu)

Experimental Methodologies to Visualize Early Cell Biology in Zebrafish Embryos,ry, respectively. If performed correctly, these techniques can provide detailed information regarding the endogenous locations of proteins and their ectopic locations or absence in mutants and in disease states.

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Vertiefung des Wissens in der Ausstellung, in aquaporin biology. This chapter focuses on techniques used for oocyte preparation and aquaporin expression and gives an overview of specific methods to determine water and solute permeability of the channels and their intracellular trafficking in oocytes.

認(rèn)識(shí)

Export Consortia in Developing Countriesescribe essential steps needed for the cryopreservation of spermatogonial stem cells (SSCs) and their utilization in the conservation of zebrafish genetic resources through SSC transplantation and surrogate production.

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