Titlebook: Genome Instability; Methods and Protocol Marco Muzi-Falconi,Grant W Brown Book 2018 Springer Science+Business Media LLC 2018 eukaryotes.tel

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Study of UV-induced DNA Repair Factor Recruitment: Kinetics and Dynamics, the site of UV-induced DNA damage..Using Isopore filters with high density pores of a broad range of sizes, it is possible to UV irradiate and damage only a very small portion of the nucleus of a cell by letting UV light pass only through the pores. Immunofluorescent analyses of modified DNA nucleo

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Inserting Site-Specific DNA Lesions into Whole Genomes,ation of a plasmid containing a site-specific lesion engineered in vitro into a precise location in the genome via the site-specific recombination reaction from phage lambda. The notion of DNA lesion is not restricted to chemically modified nucleotides but also refers to unusual DNA structures. This

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A qPCR-Based Protocol to Quantify DSB Resection,repair the break. This process is called DNA end resection and it is finely regulated to prevent genome rearrangements. Here, we describe a protocol to quantify DSB resection rate by qPCR, which could be applied to every organisms whenever the break site and its flanking region sequences are known.

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