Titlebook: Genome Editing in Animals; Methods and Protocol Izuho Hatada Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under

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https://doi.org/10.1007/978-3-8349-8779-2ryonic stem cells, an exogenous DNA sequence can be inserted into the target locus in the zygote using genome-editing technology. In this chapter, I describe the generation of epitope-tagged mice using engineered endonuclease and single-strand oligodeoxynucleotide through the mouse zygote as an exam

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https://doi.org/10.1007/978-3-8349-4748-2nding disease pathophysiology. Recently, important advances in genome editing technologies have enabled us to efficiently create sophisticated animal models in short periods of time. Base editing is a modified CRISPR/Cas system that induces base substitution at targeted genomic regions. Here I descr

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https://doi.org/10.1007/978-3-031-21274-1tegies for genotyping flox mice, typically based on Sanger sequencing following cloning of target sequences from dozens of pups, are time-consuming. Here, we describe a rapid screening method for flox mice, using in vitro Cre recombination that can be performed using simple enzymatic reactions and e

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Professionelles Desktop Publishing, after the Cas9-induced double-strand DNA breaks; the activation of the DNA repair pathway is known to be correlated with the cell cycle. Recently, we have reported a new KI approach named SPRINT (.-phase .onuclear .jection for .argeting)-CRISPR, focusing on the correlation between the cell cycle an

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Desktop Publishing — Was bringt’s wirklich?s. The intravenous injection of an AAV vector harboring the gene of interest driven by the hepatocyte-specific promoter could efficiently express the target gene in liver hepatocytes. The delivery of genome editing tools including Cas9 and gRNA, by the AAV vector, can efficiently disrupt the target

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