Titlebook: Genome Editing in Animals; Methods and Protocol Izuho Hatada Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under

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Efficient CRISPR/Cas9-Assisted Knockin of Large DNA Donors by Pronuclear Microinjection During S-Ph after the Cas9-induced double-strand DNA breaks; the activation of the DNA repair pathway is known to be correlated with the cell cycle. Recently, we have reported a new KI approach named SPRINT (.-phase .onuclear .jection for .argeting)-CRISPR, focusing on the correlation between the cell cycle an

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Genome Editing of Murine Liver Hepatocytes by AAV Vector-Mediated Expression of Cas9 In Vivo,s. The intravenous injection of an AAV vector harboring the gene of interest driven by the hepatocyte-specific promoter could efficiently express the target gene in liver hepatocytes. The delivery of genome editing tools including Cas9 and gRNA, by the AAV vector, can efficiently disrupt the target

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A Simple and Efficient Method for Generating KO Rats Using In Vitro Fertilized Oocytes,ce. However, in rats, an efficient genome editing technique that uses in vitro fertilized oocytes has not been established. Recently, we reported the stable generation of offspring from five standard strains of rats by superovulation and in vitro fertilization (IVF). Furthermore, genome-edited rats

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Editing the Genome of the Golden Hamster (,),antageous in the study of reproductive and developmental biology: a highly stable 4-day estrous cycle, a high responsiveness to conventional superovulation methods, and a shortest gestation period (16?days) known among eutherian mammals. Besides these advantages, the technical ease of in vitro ferti

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