Titlebook: Genome Editing; Current Technology A Shabir Hussain Wani,Goetz Hensel Book 2022 The Editor(s) (if applicable) and The Author(s), under excl

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978-3-031-08074-6The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerl

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https://doi.org/10.1007/978-3-031-08072-2Transgenic breeding; Genomic selection; CRISPR/Cas9; Cas endonucleases; gene targeting; biotic stress; abi

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Multiplexed Genome Editing in Plants Using CRISPR/Cas-Based Endonuclease Systemsizes strategies and the basic principles of multiplexed genome editing. It introduces the two-component transcriptional unit multiplex system, multiplexing using a single Pol III promoter, using bidirectional promoters, and highlights applications using the respective cloning procedure.

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Transgene-Free Genome Editing in Plants of any transgene, simplifying the regulatory processes in many jurisdictions around the globe. In this chapter, we present methods and delivery methods that can be used for transgene-free editing and present first promising examples.