Titlebook: Genome Editing; Kursad Turksen Book 2016 Springer International Publishing Switzerland 2016 CRISPR.ESC.iPSC.nucleases.stem cells

enmesh

哺乳動(dòng)物

escalate

CT-angiography

Developments in the Generation of Reporter Stem Cells,zed because of the associated technical difficulty; however, new advances in genome-editing technologies has lowered these barriers for creating knock-in reporter lines. This chapter provides an overview of the methodology and potential applications of reporter stem cell lines.

elucidate

Nomadic

Book 2016se. The chapters describe latest methods such as use of targetable nucleases, investigation of the non-coding genome, mouse genome editing, increasing of knock-in efficiency in mouse zygotes, and generation of reporter stem cells; the text contextualizes these methods in treatment of cardiovascular

antipsychotic

https://doi.org/10.1007/978-3-936560-43-5 to correct disease-causing mutations, modify crops and livestock, and perhaps modify our environment. This chapter discusses the advances in genome-editing technologies and their current and future applications.

hardheaded

Troubleshooting and Optimization,J or via HDR. From the first demonstration that CRISPR-Cas can be programmed to cleave DNA in 2012, the field has advanced fast and now includes examples of targeting in many model organisms as well as gene knockout or reporter hPSC lines that will aid in the production of specific cell types, such as pancreatic β-cells.

CRUDE

Pat Langdon,Jonathan Lazar,Hua Dongue as a viable treatment option for other inherited disorders across medical disciplines. This chapter highlights current ocular gene therapy approaches, clinical and preclinical experiments, and provides a case study of the bench-to-bedside personalized medicine approach taken for a novel and rare retinitis pigmentosa mutation.

Arboreal

Genome Editing with Targetable Nucleases, to correct disease-causing mutations, modify crops and livestock, and perhaps modify our environment. This chapter discusses the advances in genome-editing technologies and their current and future applications.