Titlebook: Genetic Transformation Systems in Fungi, Volume 1; Marco A. van den Berg,Karunakaran Maruthachalam Book 2015 Springer International Publis

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Transformation of , Protoplastsr their purposes. Preparation and manipulation of protoplasts has become a key step to a more efficient transformation procedure for this fungus, which is used as model species in the Mucorales to develop fermentation processes for the production of β-carotene, other carotenoids, and essential oils like gamma-linolenic acid and biodiesel.

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Electroporation of ,regg et al., Mol Cell Biol 5:3376–3385, 1985), and current methods to make chemically competent . cells results in significantly fewer transformants (. protocols, 2nd edn, Totowa, 27–42). Following are protocols to prepare E-comp . cells, and to electroporate plasmid DNA into these cells to generate transformed cells.

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2198-7777 mammalian infection (.Penicillium marneffei. and .Candida.). .Genetic Transformation Systems in Fungi, Volume 1 .provides in-depth coverage of how the transformation of DNA is used to understand the genetic basis behind these fungal traits. .978-3-319-37970-8978-3-319-10142-2Series ISSN 2198-7777 Series E-ISSN 2198-7785

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Accepting the truth of hard messagesith valuable phenotypes. These procedures should be essential tools to characterize the physiological roles of the increasing number of genes available as a result of the fungal genomic projects (.) (Grigoriev et al., Nucleic Acids Res 40:D26–32, 2012).

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Fungal Transformation: From Protoplasts to Targeted Recombination Systemst fungi impaired in the NHEJ system allowed to select recombinants at homologous sites with high efficiency. These advances have contributed significantly to the progress in the molecular biology of fungi.

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