Titlebook: Genetic Library Construction and Screening; Advanced Techniques R. Curtis Bird,Bruce F. Smith Book 2002 Springer-Verlag Berlin Heidelberg

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Brentanos Versuch der Grundlegung der Ethik,ssion, modification or further subcloning. A wide variety of strategies have been employed and are available in commercially supplied kits (see Page 30 for a listing of manufacturers of PCR cloning kits or vectors). These approaches include simple but relatively inefficient blunt ligation schemes th

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Das Problem der Industriearbeiting numerous living organisms or cells for naturally existing mutants, often following exposure to a mutagen. Since DNA is reproduced in vivo quite faithfully according to its template and most errors are efficiently detected and reversed by DNA repair systems, naturally occurring mutations are rare

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https://doi.org/10.1007/978-3-322-98641-2nces for target identification, sequence determination, subsequent use as molecular probes, or as functional units which can be expressed in appropriate vector systems. This chapter describes the technique of Long RT-PCR followed by cloning of the PCR products into plasmid vectors. Essentially, this

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https://doi.org/10.1007/978-3-642-90660-2on, the transcripts that are newly transcribed as a result of altered physiology. Whether in response to a natural phenomenon such as a growth factor or morphogen stimulation, or in response to environmental stimulation or stress, new transcripts appear as a result of newly stimulated gene expressio

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978-3-642-47733-1Springer-Verlag Berlin Heidelberg 2002

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