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,Isolation of Intact mRNA and Construction of Full-Length cDNA Libraries: Use of a New Vector, λgt22y to isolate cDNA representing a complete copy of an intact mRNA, especially when it is to be cloned from a tissue rich in nuclease or when the mRNA is very large (more than 5 kb). Recently we have constructed a cDNA library from adult rat pancreas and isolated cDNA clones which encode the lysophosp

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https://doi.org/10.1007/978-3-322-80281-1fs within promoter and enhancer elements. By unknown mechanisms they contribute to the observed transcriptional specificity with regard to cell type, developmental timing and environmental responsiveness (1–3). Many of these DNA binding activities can be assayed in crude nuclear extracts (4,5) after

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Das Framing von Issues in Medien und Politikmined nucleotide sequence and are now wondering which theoretical methods and associated computer programs you should use to glean structural and functional information from the mere sequence of letters.”

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Erfahrungen deutscher Firmen in Frankreich,portant tools in the arsenal of techniques used to analyze gene regulation are reporter genes, which, when linked to appropriate regulatory elements, provide insights into the levels of gene expression and the DNA segments that modulate this expression. In addition, such genes may also be utilized t

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https://doi.org/10.1007/978-3-8349-9973-3his situation has changed drastically with the advent of automated methods of oligonucleotide synthesis in combination with rapid sequencing and cloning techniques (2). Now, with the technique of oligonucleotide-directed mutagenesis specific and direct manipulations at the level of a single base in

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https://doi.org/10.1007/978-3-663-04546-5rmination (1). In spite of its importance -- E. B. Wilson (2) called it “the fundamental problem...which includes all others” -- determination has received far less attention from molecular biologists than has cell differentiation. One reason may be that determination is not a visible property of ce

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