Titlebook: Generation of cDNA Libraries; Methods and Protocol Shao-Yao Ying Book 2003 Humana Press 2003

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cDNA Library Construction Using In Vitro Transcriptional Amplification,he promoter element of the cDNA to synthesize the aRNA sequence up to 2000-fold increase, (4) reverse transcription of the aRNA, (5) denaturation and then double-stranding the resulting cDNA with promoter-linked oligo-(dT) primers, and (6) repeating steps 3–5 to achieve the desired cDNA or aRNA amount for the library preparation.

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Quality Assessment of cDNA Libraries,h-quality data, including valid sequence information, rigorous quality assessment is also needed after library construction (i.e., prior to and during the use of a library for downstream applications). Applying a set of common quality criteria to newly generated cDNA libraries will become increasingly important (.).