Titlebook: Gene Transfer and Expression Protocols; E. J. Murray Book 1991 Springer Science+Business Media New York 1991

BOLT

CLAIM

Merited

放逐某人

Calcium Phosphate Mediated Gene Transfer into Established Cell Lines,DNA transfection is one of the most important techniques in molecular genetics. It is this technique that has made possible the dissection of complex eukaryotic genes and the characterization of the function of their components (.–.) as well as the isolation of particular genes on the basis of their expression in cultured cells (.–.).

Cardiac-Output

Arteriography

Transfection of the ChloramphenicolAcetyltransferase Gene into Eukaryotic Cells Using Diethyl-Aminoimply, a way of getting DNA into a cell; and second, a reporter system, which, as the name suggests, is a means of finding out what the transfected DNA does from its new location inside the cell. These two requirements are amply met by transfecting cells, in the presence of the polycation diethyl am

發(fā)怨言

Polybrene/DMSOAssisted Gene Transfer, displayed by the recipient cells toward the gene-transfer regimen itself. As a result, several laboratories have turned to the use of synthetic polycations for delivering copious amounts of exogenous DNA to target cells without compromising their viability or clonogenicity. One of these compounds,

種類

Electroporation Technique of DNA Transfection,on of cells and DNA placed between electrodes in a suitable cuvet. It is thought that this pulse induces local areas of cell-membrane breakdown, or pores, through which the DNA then enters the cell. Once these pores have resealed, normal cell functions can continue. Since this transfection method in

MOTIF

Antagonist

,Direct Use of λ Phage Particles for DNA Transfection,m other mammalian cells, and also the study of the function and the regulation of gene expression of mammalian genes in vivo. Various transfection techniques have been developed: incubation of recipient cells with genes in the presence of diethylaminoethyldextran (.., this vol.; ref. .); incubation