Titlebook: Gene Transfer and Expression Protocols; E. J. Murray Book 1991 Springer Science+Business Media New York 1991

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https://doi.org/10.1007/978-1-4615-5013-6n cells. This technique, termed chromosome-mediated gene transfer (CMGT), involves the transfer of subchromosomal fragments from the cells of one species (the donor, usually human) to those of another (the recipient, usually a rodent) that have the ability to maintain them. CMGT thus fills a gap bet

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Akira Hashizume,Naruhito Hironagae 43 of them human. The human adenoviruses, particularly types 2, 5, and 12, have been the most extensively characterized, and these viruses have served as valuable tools in the study of the molecular biology of DNA replication, transcription, RNA processing, and protein synthesis in mammalian cells

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Clinical Applications of Medical Imaging >50 different virus gene products (for reviews, .refs. .). The widespread use of vaccinia virus as a vector owes much to the ease of generating recombinants, the authenticity of the foreign gene product, and the wide variety of uses to which they have been put. For example, HIV 1 (human immunodefic

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https://doi.org/10.1007/978-1-4613-1573-5sizing foreign gene products in a heterologous system. In contrast to the other recent excellent reviews on the subject (.,.), this contribution aims to explore the various practical aspects of the system, and in consequence, to highlight particular difficulties that may be encountered by workers ne

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Diagnosis and Treatment of Hyperthyroidismled DNA molecule of 5243 bp (.) that is packaged into a capsid containing the viral structural proteins VP1,VP2, and VP3. SV40 induces a lytic infection cycle in permissive cells. The first step in the replication cycle is synthesis of early mRNA. This is followed by DNA replication and expression o

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RET and Some Mid-Life Problems,panded the capabilities of investigators to perform important experiments that have solved previously unanswerable biological questions. Retroviral vectors exploit the inherent capacity of retrovkuses to transfer genetic material stably into a cell’s genome and subsequently express it in a manner th

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I-MIBG SPECT/CT for Tumour Imaging, use of reporter genes. In essence, a reporter gene encodes a protein product for which a sensitive and convenient assay is available. Transcription of the reporter is driven by the control sequences of interest, so that the level of reporter protein reflects the transcriptional activity, Reporter g

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Physics and Technology of SPECT/CT,nto heterologous cellular systems both in vitro and in vivo. One approach to defining DNA sequences important in the regulation of gene expression is to place controlling elements (e.g., promoter/enhancer sequences) upstream of a DNA coding sequence, introduce these constructs into transgenic animal