Titlebook: Gene Therapy Protocols; Paul D. Robbins Book 19971st edition Springer Science+Business Media New York 1997

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John P. Houghton,Andrew Stevensive way of delivering genes into mammalian cells. This chapter aims to explore the various practical aspects of the AAV vector system, and in consequence, to highlight particular difficulties that may be encountered by workers new to the field. However, before describing the methodology involved in

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https://doi.org/10.1057/9781137387967ewed in ref. .). One serious drawback of this technique, however, has been the difficulty in readily producing high-titer recombinant retroviruses. For many applications, such as infecting rare target cells or the majority of cells in tissue culture, the recombinant virus titer must be at least 10.

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https://doi.org/10.1007/978-3-030-10847-2iently infect mammalian cells, the potential risks of viral gene delivery spurred research in the development of synthetic chemical vectors that would duplicate viral delivery but have no risk of infectious complications. These nonviral vectors were first developed in the late 1980s. Cationic lipids