Titlebook: Gene Mapping, Discovery, and Expression; Methods and Protocol Minou Bina Book 2006 Humana Press 2006 Allele.DNA.Microarray.Promoter.gene ex

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Use of Genome Browsers to Locate Your Favorite Genes,ic example, this chapter provides a step-by-step procedure for creating a map using the genome browser at UCSC. The example describes mapping, in the human genome, the promoter region of the NF-IL6 gene. The procedure is applicable to creating maps of the desired regions in genomes of other species available at the genome browser at UCSC.

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Computational Prediction of cis-Regulatory Modules from Multispecies Alignments Using Galaxy, Table as high conservation score, high regulatory potential score, and conserved transcription factor binding sites. Links to these servers can be accessed at http:// www.bx.psu.edu/ and http://genome.ucsc.edu/.

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Comparative Promoter Analysis in Vertebrate Genomes With the CORG Workbench,otate promoter regions. Second, experimental data on transcription start sites: exon positions and DNA binding site descriptions complete the promoter annotation. These data are made available via an interactive web portal. Individual promoter studies are supported by a JAVA applet that supplies all data down to the nucleotide level.

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A Database of 9-Mers from Promoter Regions of Human Protein-Coding Genes,ures of potential regulatory “words,” we have created a database of 9-mers derived from the promoter regions of a subset of human protein-coding genes. This report describes the procedure for extracting information from that database through the web.

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New Findings Concerning the Initial Lesion,sualized by fluorescence microscopy in metaphase spreads from patients and controls. With further development, the PRINS method may prove useful for localization of single-copy genes, in general, and for the detection of gene deletions.