Titlebook: Gene Knockout Protocols; Wolfgang Wurst,Ralf Kühn Book 2009Latest edition Humana Press 2009 DNA.ES cells.Gene modification.Genetically eng

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Differentiation Analysis of Pluripotent Mouse Embryonic Stem (ES) Cells In Vitro type of the organism. Here we describe basic protocols for the in vitro differentiation of mouse ES cells into cells of the cardiac, neuronal, pancreatic, and hepatic lineage. The protocols include (1) the formation of embryoid bodies (EBs) followed by (2) the spontaneous differentiation of EBs int

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Cloning of ES Cells and Mice by Nuclear Transferiezo-actuated micromanipulator. Although the piezo unit is a complex tool, once mastered it is of great help not only in NT experiments, but also in almost all other forms of micromanipulation. Using this technique, embryonic stem (ntES) cell lines established from somatic cell nuclei can be generat

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Aggregation Chimeras: Combining ES Cells, Diploid, and Tetraploid Embryosrom undifferentiated cell populations to fully committed functional cell types. This chapter gives a description of the early events of cell commitment and differentiation in the pre-and postimplantation-stage embryo. Next, a discussion follows highlighting the most commonly used as well as more rec

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VelociMouse: Fully ES Cell-Derived F0-Generation Mice Obtained from the Injection of ES Cells into Er equally high-throughput methods for the production of mice for phenotypic studies. In response to this challenge, we recently developed a new method termed VelociMouse for the production of F0-generation mice that are fully derived from gene-targeted ES cells. In the version of the VelociMouse met

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Book 2009Latest editiong of their molecular interaction network. The mouse offers many advantages for the use of genetics to study human biology and disease, unmatched among other m- mals. Its development, body plan, physiology, behavior, and diseases have much in common, based on the fact that 99% of the human genes have

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