Titlebook: Gene Expression Analysis; Methods and Protocol Nalini Raghavachari,Natàlia Garcia-Reyero Book 2018 Springer Science+Business Media, LLC, pa

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https://doi.org/10.1007/978-1-4939-7834-2exportin-5; nucleocytoplasmatic transport factors; chromatin modiification; histone acetylation; cytosin

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Nalini Raghavachari,Natàlia Garcia-ReyeroIncludes cutting-edge methods an protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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RNA-Seq and Expression Arrays: Selection Guidelines for Genome-Wide Expression Profiling,ore the transcriptome and to better understand biological systems and their perturbation. In this chapter we provide an overview of microarray and massively parallel sequencing technologies and their application to gene expression analysis. We discuss factors that impact expression data generation a

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,Simultaneous, Multiplexed Detection of RNA and Protein on the NanoString? nCounter? Platform,n (mRNA), DNA, and protein. The technology uses molecular barcodes and single-molecule imaging to detect and count unique mRNA and protein targets in a single reaction. nCounter-based detection is enzyme-free (no amplification of mRNA is required), fully automated, and allows simultaneous detection

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Transcript Profiling Using Long-Read Sequencing Technologies,arly for large-scale high-throughput studies. NGS technologies comprise short-read RNA-Seq (dominated by Illumina) and long-read RNA-Seq technologies provided by Pacific Bioscience (PacBio) and Oxford Nanopore Technologies (ONT). Although short-read sequencing technologies are the most widely used,