Titlebook: Gene Essentiality; Methods and Protocol Long Jason Lu Book 2015 Springer Science+Business Media New York 2015 Candida albicans.Computationa

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A Coastal Classroom without Walls,enes in the . SK36 strain using genome-wide deletion mutation. A fusion PCR-based method is used to construct gene deletion fragments, which contain kanamycin resistance cassettes with two flanking arms of DNA upstream and downstream of the target gene. The linear fused PCR amplicons were transforme

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