Titlebook: Gene Delivery to Mammalian Cells; Volume 1: Nonviral G William C. Heiser Book 2004 Humana Press 2004 DNA.Ethylen.Vivo.gene transfer.genes.l

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DNA Delivery to Cells in Culture Using Cationic Liposomesmonstrated to react spontaneously with anionic DNA to form lipid-DNA complexes, or “l(fā)ipoplexes” (.). These lipoplexes demonstrated effective binding to cells in vitro and facilitation of intracellular delivery of a transgene. Felgner provides a detailed history of the progress leading up to and including lipofection (.).

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Delivery of DNA to Cells in Culture Using Particle Bombardmentmammalian cells in vitro, in this chapter I describe protocols using the PDS-1000/He. In experiments comparing gene expression in cells bombarded using both instruments, expression is routinely two- to three-fold higher in cells bombarded using the PDS-1000/He (unpublished results).

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Delivery of DNA to Skin by Particle Bombardmentd the Helios gene-gun system commercially available at Bio-Rad Laboratories. In this chapter, the Bio-Rad gene-gun system is used as a model to describe the delivery of DNA to skin by particle bombardment and the parameters affecting such gene transfer.

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William C. HeiserIncludes supplementary material:

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