Titlebook: Gene Correction; Methods and Protocol Francesca Storici Book 2014 Springer Science+Business Media, LLC 2014 gene correction.gene repair.gen

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Designing and Testing the Activities of TAL Effector Nucleasestime-consuming experimental screening for active TALENs, a scoring system can help select optimal target sites. Here we describe a procedure to design active TALENs using a scoring system named Scoring Algorithm for Predicted TALEN Activity (SAPTA) and a method to test the activity of individual and

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Design and Analysis of Site-Specific Single-Strand Nicking Endonucleases for Gene Correctiond break-producing enzymes, and nickases have been engineered from both homing endonuclease and .I-based scaffolds. We describe the strategies used to engineer these site-specific nickases as well as the in vitro methods used to confirm their activity and specificity. Additionally, we describe the Tr

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Book 2014enetic variants. .Gene Correction: Methods and Protocols .provides a user friendly, detailed and up-to-date collection of strategies and methodologies utilized for generating specific sequence changes in the DNA of cells in the laboratory, while also tackling the major problems that the field of gen

aerial

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https://doi.org/10.1007/978-3-0348-7985-9get location. Cells transformed with the substrate undergo homologous recombination between the genomic DNA and the recombineering substrate. The recombinants are found by selection for traits carried by the recombineering substrate, usually antibiotic resistance.

archetype

https://doi.org/10.1007/978-3-0348-9381-7ineering to conduct targeted modifications of individual loci. Here we describe the recombineering designs and procedures for the introduction of epitope tags, in-frame deletion mutations, and point mutations into plasmids that can later be used for SIRT.

Discrete

https://doi.org/10.1007/978-1-4684-1233-8 used to isolate derivatives of the I-SceI homing endonuclease from combinatorial libraries that display altered DNA recognition specificities. The construction of plasmid expression libraries, the development of reporter strains, and the utilization of these components in the bacterial one-hybrid system are detailed.