Titlebook: Gel-Free Proteomics; Methods and Protocol Kris Gevaert,Jo?l Vandekerckhove Book 2011 Springer Science+Business Media, LLC 2011 LPI hexalene

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Metabolic Labeling of Model Organisms Using Heavy Nitrogen (15N),healthy vs. diseased”), that have received different treatments, or that have different genetic backgrounds. Protein expression levels can be quantified by labeling proteins with stable isotopes, followed by mass spectrometric analysis. Stable isotopes can be introduced in vitro by reacting proteins

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ICPL Labeling Strategies for Proteome Research,plex protein mixtures. Isotope-coded protein label (ICPL) is capable of high-throughput quantitative proteome profiling on a global scale. Since ICPL is based on stable isotope tagging at the free amino groups of intact proteins, it is applicable to any protein sample, including extracts from tissue

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Quantitative Proteome Analysis Using Isobaric Peptide Termini Labeling (IPTL),. We have recently introduced isobaric peptide termini labeling (IPTL), a novel strategy for isobaric quantification based on the derivatization of peptide termini with complementary isotopically labeled reagents. Unlike non-isobaric quantification methods, sample complexity at the MS level is not i

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Complete Chemical Modification of Amine and Acid Functional Groups of Peptides and Small Proteins,metry (MS). Modification at other functional groups has received less attention in MS-based proteomics. Amine modification (Lys, N-termini) by reductive dimethylation or by acylation (e.g., iTRAQ labeling) has recently gained some popularity in peptide-based approaches (bottom-up MS). Modification a

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,Membrane Protein Digestion – Comparison of LPI HexaLane with Traditional Techniques,es. Traditional techniques used for soluble proteins, such as 2D gel electrophoresis, are sometimes not entirely applicable to membrane protein targets, due to their low abundance and hydrophobic character. New tools have been developed that will accelerate research on membrane protein targets. Lipi

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