Titlebook: Gastrointestinal Physiology and Diseases; Methods and Protocol Andrei I. Ivanov Book 2016 Springer Science+Business Media New York 2016 gas

Meditative

Imaging Inflammatory Hypoxia in the Murine Gut In ulcerative colitis, inflammation tends to be more superficial and restricted to the colon; contrastingly, Crohn’s disease presents as patchy, more penetrative inflammation that can occur throughout the gastrointestinal tract. Other differences between these diseases include the nature of their r

Handedness

Label-Free Imaging of Eosinophilic Esophagitis Mouse Models Using Optical Coherence Tomography such as feeding difficulties and food impaction. The pathophysiological mechanisms underlying EoE remain poorly understood. Preclinical studies using mouse models have been critical in comprehending human disease mechanisms and associated pathways. In this chapter, we describe an experimental metho

Obverse

Near-Infrared Fluorescence Endoscopy to Detect Dysplastic Lesions in the Mouse Colonudies, we developed a small animal NIRF endoscope and successfully used this device to identify dysplastic lesions in a murine model of chronic colitis. In this chapter, we present a step-by-step protocol for using NIRF endoscopy to examine the location, the size, and the borders of the dysplastic l

Infantry

Visualization of Signaling Molecules During Neutrophil Recruitment in Transgenic Mice Expressing FREmical mediator on the intracellular signaling networks controlling cell migration have been studied with neutrophils cultured in vitro, how such chemical mediators act cooperatively or counteractively in vivo remains largely unknown. To understand the mechanisms regulating neutrophil recruitment to

內(nèi)向者

In Vivo Myeloperoxidase Imaging and Flow Cytometry Analysis of Intestinal Myeloid Cellsand inflammatory monocytes. While lacking specificity for any of these three cell types, MPO imaging can provide guidance for further flow cytometry-based analysis of tissues where these cell types reside. Isolation of leukocytes from the intestinal tract is an error-prone procedure. Here, we descri

novelty

Macrophage Isolation from the Mouse Small and Large Intestinepathogens. One of the most important steps in beginning to understand the functions of these cells is the ability to effectively isolate them from the complex intestinal environment. Here, we detail methodology for the isolation and phenotypic characterization of macrophages from the mouse small and

有害

yohimbine

1064-3745 tips ontroubleshooting and avoiding known pitfalls..Highly practical and clearly written,?.Gastrointestinal Physiology andDiseases: Methods and Protocols.?will serve both seasone978-1-4939-8091-8978-1-4939-3603-8Series ISSN 1064-3745 Series E-ISSN 1940-6029

neutral-posture

Lentivirus-Based Stable Gene Delivery into Intestinal Organoidsr while remaining attached to the matrix. Using a GFP-labeled lentivirus, we demonstrate that this method allows for highly efficient infection of intestinal organoids after overnight incubation of Matrigel-attached cells with lentiviral particles.

干涉

Imaging Inflammatory Hypoxia in the Murine Gute HIF (hypoxia inducible factor) transcription factor, which regulates adaptive pathways including genes controlling glycolytic metabolism and angiogenesis. In adequately oxygenated tissues (i.e. normoxia), the HIF protein is constantly produced, but oxygen-dependent enzymes called prolyl-hydroxylas