Titlebook: Galectins; Methods and Protocol Sean R. Stowell,Richard D. Cummings Book 2015 Springer Science+Business Media New York 2015 galectin funct

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Philippe Malaval,Christophe Bénaroyasensitivity and low sample consumption in the BIAcore enables the measurement of rapid kinetics and low affinities characteristic of many biological interactions. This chapter describes the affinity measurement of Galectins-1, -2, and -3 and their glycoside ligands using this approach.

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Aerosol Optical Depth and Precipitationtin family is also defined by amino acid homology within structural domains and exon/intron junctions within genes. As galectins are expressed by organisms as diverse as some fungi, ., fish, birds, and mammals, and biological activities attributed to galectins are equally diverse, it becomes essenti

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Leaven

Jacqueline Lenoble,Maurice Hermanstant, binding stoichiometry, and heat of binding (enthalpy) and indirectly the entropy and free energy of binding. ITC has been extensively used to study the binding of lectins to mono- and oligosaccharides, but limited applications to lectin–glycoprotein interactions. Inherent experimental challen

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Philippe Malaval,Christophe Bénaroyasensitivity and low sample consumption in the BIAcore enables the measurement of rapid kinetics and low affinities characteristic of many biological interactions. This chapter describes the affinity measurement of Galectins-1, -2, and -3 and their glycoside ligands using this approach.

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,Inertial Force Sensors — Accelerometers,le cell surface glycans. While many studies demonstrate the impact of glycan modification on GBP recognition and activity, the relative contribution of subtle changes in glycan structure on GBP binding can be difficult to define. To overcome limitations in the analysis of GBP-glycan interactions, re