Titlebook: G Protein-Coupled Receptors in Drug Discovery; Methods and Protocol Marta Filizola Book 2015Latest edition Springer Science+Business Media

綁架

cruise

EVADE

Detection and Quantification of Intracellular Signaling Using FRET-Based Biosensors and High Content FRET biosensors to measure cAMP levels, kinase (ERK and PKC), and GTPase activity. Importantly, we provide the protocols to express and measure these sensors in a variety of model cell lines and primary dorsal root ganglia neurons.

生意行為

多產(chǎn)子

Bioluminescence Resonance Energy Transfer Approaches to Discover Bias in GPCR Signaling,e molecules of interest. As BRET is readily applied to the study of numerous GPCR signaling and regulatory paths, it is an ideal technique for investigating the pharmacology of biased ligands and receptors.

Ataxia

Radioligand Binding Assay for an Exon 11-Associated Mu Opioid Receptor Target,ciated with exon 11. These exon 11-associated truncated variants are not readily labeled with current radioligands. Here we describe the synthesis of a radioiodinated ligand suitable for carrying out binding studies for this target.

巫婆

壓艙物

Mirage

Regular Press Conference (July)g of GPCRs with bright organic fluorophores and fluorescent imaging by total internal reflection fluorescence microscopy. Using this method, individual tracks of single molecules can be analyzed in parallel with high spatial precision and with frame rates up to 50/s.

beta-cells

https://doi.org/10.1007/978-3-030-75401-3 application to determine not only protein–protein interactions but also the capability of GPCR mutant variants to form homomers compared to the wild type GPCR within the plasma membrane of transfected cells.